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- W2365676372 abstract "Objective Toestablisham et hodofisolating and culturing dendritic cells (DCs) from the bone marrow of F344 rats.Methods Dendritic cells obtained from the bone marrow ofF344rat′slimbsbysaline lavationwereculturedintheRPMI 1640 in vitro under the condition of granulocyte-ma crophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4).Dendrit ic cells were purified by centrifugation and identified by inversion microscopy, electron microscopy and flow cytometry.Resu lts After culture and induction,DCs displayed typical form with elongat ed dendritic processes viewed by inversion microscopy and electron microscopy.DC s expressed surface antigens,including OX62 8.92%,MHC-Ⅱ 20.9%,CD86 16.98% on t he 4th day;OX62 58.07%,MHC-Ⅱ 60.49%,CD86 62.94% on the 8th day;OX62 84.68%, MHC-Ⅱ 88.03%,CD86 62.80% on the 15th day.Conclusion The purity of DCs is up to 80% and over using this mothod,which presents the feasibility for further clinical use of DC in tumor immunotherapy." @default.
- W2365676372 created "2016-06-24" @default.
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- W2365676372 date "2005-01-01" @default.
- W2365676372 modified "2023-09-25" @default.
- W2365676372 title "Isolation and culture of F344 rat′s dendritic cells" @default.
- W2365676372 hasPublicationYear "2005" @default.
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