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- W2365681539 abstract "The three expressing plasmids of pcDNA3.1 (-)CVyCDglyTK, pcDNA3.1 (-)CEAyCDglyTK, pcDNA3.1 (-)CMV-yCDglyTK were constructed with carcino-embryonic antigen (CEA) promoter and cytomegalovirus (CMV), CEA promoter, cytomegalovirus (CMV) enhancer, respectively. Calcium phosphate nanoparticles (CPNP) was used as vector to transfer the three plasmids into CEA-positive cells (Lovo) and CEA-negative cells (HeLa), respectively. The results showed that expression of yCDglyTK mRNA in all cells transferred by the three plasmids above, which significantly sensitized the cytotoxicity of prodrug 5-FC; expression of yCDglyTK mRNA in HeLa cells after transfected by CPNP-pCDNA3.1 (-)CMVyCDglyTK, which enhanced sensitivity of 5-FC, but the other plasmids had no expression of yCDglyTK mRNA and 5-FC had no killing effect. pcDNA3.1 (-)CVyCDglyTK might be a promising candidate vector for colon carcinoma gene therapy." @default.
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- W2365681539 date "2009-06-30" @default.
- W2365681539 modified "2023-09-27" @default.
- W2365681539 title "Construction of Calcium Phosphate Nanoparticles Mediating Fusion Suicide Gene yCDglyTK and its Application in vitro" @default.
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