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- W2365698525 abstract "Objective To select B cell epitopes of calpain and to explore antigenicity of the B cell epitope and immune respose type induced by the B cell epitopes. Methods Hydrophilicity of predicted calpain amino acid sequence was analyzed by Genetyx-MAC9.0. Overlapping 9-mer oligopeptides spanning hydrophilic portions of residues 229-375 and 555-613 were synthesized in solid phase by Auto-spot Robot. Dot-ELISA to detect liner epitopes for B cell epitopes in synthesizing peptides library. BALB/c mice were immunized by B cell epitope, IgG antibodies subclass of immunized mice were detected by ELISA. Results Two major hydrophilic portions of Schistosoma japonicum(S.japonicum) calpain were predicted which located at residues 229-375 and 555-613. There were four common B cell epitopes in schistosomal calpain as 234-251(YAHLSGGT); 290-297(CLMGCSIH); 338-346(PWGDSHEW); 358-364(AWCDGAPQ); After 4 weeks of being immunized with B cell epitopes, IgG1, IgG2a and IgG2b antibodies significantly increased in sera of immunized mice compared with control ones. Conclusion Together with these results, calpain of S.japonicum indicated strong antigenicity which seems to be not only a vaccine candidate, but also a target antigen for immuno diagnosis of human schistosomiasis." @default.
- W2365698525 created "2016-06-24" @default.
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- W2365698525 date "2002-01-01" @default.
- W2365698525 modified "2023-09-25" @default.
- W2365698525 title "Prepareration of peptides library and selection of B cell epitopes of Schistosoma japonicum calpain" @default.
- W2365698525 hasPublicationYear "2002" @default.
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