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- W2365698911 abstract "Objectives:To express the outermembrane segment of mouse CTLA-4 in eukaryotic cells.Study the peptide's binding to B7 of antigen present cells could rescue the T cells activation after stimulated,and therefor promote the T cells proliferation.Methods:The total RNA were purified from mouse spleen cells.By RT-PCR the whole gene of CTLA-4 was amplified.Primers designed according to the pcDNA3.1 and CTLA-4 outer membrane sequences,and employed for PCR.Amplified sequence together with the plasmid were cut and reconstructed.The right plasmid was further transferred into Hepa1-6 cells,and the expression was examined by western-blot.Results:CTLA-4 outer membrane segment expressing Hepa 1-6 cells were achieved.The expressed peptide were positive to anti-His antibody and CTLA-4 antibodies on western blot.Conclusion:The recombinant peptide were obtained and will be applied for cell and animal expreiments." @default.
- W2365698911 created "2016-06-24" @default.
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- W2365698911 date "2010-01-01" @default.
- W2365698911 modified "2023-09-26" @default.
- W2365698911 title "Eukaryotic Expression and Purification of Mouse CTLA-4 Outermembrane Segment" @default.
- W2365698911 hasPublicationYear "2010" @default.
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