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- W2365707121 abstract "Objective To analyze the expression of SPACA4 in human and mice. Methods Testes cDNA samples from Balb/C mice of different postnatal days were performed with mouse Affymetrix chip to screen the expression of SPACA4 in mice. Sub-quantitative RT-PCR and bioinformatic tools were used here to describe the expression profile of SPACA4 in mice and human. Results The results of gene chip analysis indicated that the expression of mSPACA4 began after 35 of postnatal testis in mice. Sub-quantitative RT-PCR assay showed that SPACA4 gene ex- pressed exclusively in mouse and human testis, and mouse mSPACA4 gene was expressed after day 35 of postnatal testis that is consistency with the results of gene chip analysis. By bioinformatics analysis, mSPACA4 is located in cell membrane (34.8%) or plasma membrane (34.8%), the signal peptide cleavage site between position 19 and 20 amino acids, transmembrane region between 2-20 and 101-126 amino acids, respectively, on mSPACA4 protein. Conclusion mSPACA4 and hSPACA4 were testis-specific genes, and the expression of mSPACA4 begins after day 35 of postnatal testis in mice. SPACA4 is a candidate for targeting in a sperm-based contraceptive vaccine." @default.
- W2365707121 created "2016-06-24" @default.
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- W2365707121 date "2007-01-01" @default.
- W2365707121 modified "2023-09-23" @default.
- W2365707121 title "Expression and bioinformatics analysis of SPACA4 in mice and human" @default.
- W2365707121 hasPublicationYear "2007" @default.
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