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- W2365718602 abstract "Internal transcribed spacer(ITS) sequences of rDNA from silk sorghum as a quarantine weed and related species were obtained.By restriction endonuclease digestion,restriction enzyme XceⅠcut the PCR product.The sequence alignment showed that only 16 base pairs differences were found in ITS(ITS1,5.8S RNA,ITS2).It indicated that rDNA ITS region was relatively stable among the intrapopulations of 6 related species.The fragments of rDNA ITS-PCR were digested with XceⅠ.The difference between silk sorghum and its related species was found according to the pattern of digestion by XceⅠ.That is,only two bands in 670,190 bp were observed in the lanes of silk sorghum while the four fragments in 670,530,190 and 150 bp for other related species.The results were consistent with the fact that S.halepense,S.propinguum,S.propinguum(Mingfu No.1),S.almum and S.sudanense each had two selective identify sites of PmaCⅠ while S.silk had one site.Therefore,silk sorghum can be distinguished by the PCR-RFLP method established in this study." @default.
- W2365718602 created "2016-06-24" @default.
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- W2365718602 date "2005-01-01" @default.
- W2365718602 modified "2023-09-23" @default.
- W2365718602 title "rDNA ITS gene of silk sorghum by RFLP" @default.
- W2365718602 hasPublicationYear "2005" @default.
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