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- W2365720226 abstract "Objective To predict the pathway of synthesis and assembly of LPS for L.interrogans,and analyze the characterization of several involved important genes.Methods Using on-line database NCBI/Blast,ProDom/Blast,Jpred2,TMHMM et al to annotate genes on the chromosomes of L.interrogans,and using local software Bioedit and Pep Tool to analyze its characterization throughly.Results After Lipid-A precursor synthetical genes(lpxA,lpxB,lpxC,lpxD,lpxK,kdsA,kdsB,kdtA,lnt,htrB) were analyzed,as well as O-antigen synthetical rfb locus and its assembly genes (rfbP,rfbX,rfc,rfaL,rfe),the sketch map of LPS synthical and assembly pathway were finished,the secondary structure,transmembrane region,domain of the protein coded by genes:LpxA,Rfc,RfbX and RfaL were analyzed.Conclusion The pathway of synthesis and assembly for LPS in L.interrogans is similar to that of standard Gram-negative bacterium such as E.coli K-12.The difference was in metabolism of fatty acid and O-antigen structure.The annotation of LPS correlative genes using bioinformatics lay a foundation to further study on their function and relationship between LPS and pathogenesis by Leptospira." @default.
- W2365720226 created "2016-06-24" @default.
- W2365720226 creator A5032981547 @default.
- W2365720226 date "2004-01-01" @default.
- W2365720226 modified "2023-09-24" @default.
- W2365720226 title "Pathway and related genes of lipopolysaccharide biosynthesis and assembly in Leptospira" @default.
- W2365720226 hasPublicationYear "2004" @default.
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