Matches in SemOpenAlex for { <https://semopenalex.org/work/W2365720654> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2365720654 endingPage "163" @default.
- W2365720654 startingPage "160" @default.
- W2365720654 abstract "AIM To construct and screen the specific Fab phage antibody library against human Raji cell strain in B-lymphoma. METHODS BALB/c mice were immunized with Raji cells, and the antibody light chain kappa genes and heavy chain genes Fd from the spleen cells were amplified by RT-PCR. After restrictive digestion with Sac I/Xba I and Xho I/Spe I, the light chain kappa genes and heavy chain genes Fd were inserted into the phagemid vector pComb3H-SS successively and then electroporated into E.coli XL1-Blue. The specific Fab phage antibody library against Raji cell strain in human B-lymphoma was constructed by infection of helper phage VCSM13. The specific antibodies against Raji cells were obtained after selected with Raji cells. The binding activity with antigens was identified by ELISA and the positive clones were sequenced. RESULTS The Fab phage antibody library with 2.18 x 10(7) volume was constructed and eight positive clones which specifically recognized Raji cell strain were isolated. Sequence analysis of the two positive clones showed that the variable heavy domains (VH) and variable light domains (VL) were highly homologous with the registered murine Ig heavy chain V region sequences and kappa light chain sequences, respectively. CONCLUSION Fab phage antibody library was successfully constructed and specific antibodies against membrane antigens in Raji cells were obtained, which will provide an experimental foundation for the further investigation of B-lymphoma immunotherapy." @default.
- W2365720654 created "2016-06-24" @default.
- W2365720654 creator A5006668200 @default.
- W2365720654 creator A5018254630 @default.
- W2365720654 creator A5024242005 @default.
- W2365720654 creator A5060575718 @default.
- W2365720654 creator A5060805738 @default.
- W2365720654 creator A5074241483 @default.
- W2365720654 date "2007-02-01" @default.
- W2365720654 modified "2023-09-23" @default.
- W2365720654 title "Construction and screening of the specific Fab phage antibody library against Raji cell strain" @default.
- W2365720654 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17286911" @default.
- W2365720654 hasPublicationYear "2007" @default.
- W2365720654 type Work @default.
- W2365720654 sameAs 2365720654 @default.
- W2365720654 citedByCount "1" @default.
- W2365720654 countsByYear W23657206542014 @default.
- W2365720654 crossrefType "journal-article" @default.
- W2365720654 hasAuthorship W2365720654A5006668200 @default.
- W2365720654 hasAuthorship W2365720654A5018254630 @default.
- W2365720654 hasAuthorship W2365720654A5024242005 @default.
- W2365720654 hasAuthorship W2365720654A5060575718 @default.
- W2365720654 hasAuthorship W2365720654A5060805738 @default.
- W2365720654 hasAuthorship W2365720654A5074241483 @default.
- W2365720654 hasConcept C10079403 @default.
- W2365720654 hasConcept C104317684 @default.
- W2365720654 hasConcept C147483822 @default.
- W2365720654 hasConcept C153911025 @default.
- W2365720654 hasConcept C159047783 @default.
- W2365720654 hasConcept C159654299 @default.
- W2365720654 hasConcept C16232356 @default.
- W2365720654 hasConcept C186268636 @default.
- W2365720654 hasConcept C2776441376 @default.
- W2365720654 hasConcept C36394416 @default.
- W2365720654 hasConcept C542903549 @default.
- W2365720654 hasConcept C54355233 @default.
- W2365720654 hasConcept C547475151 @default.
- W2365720654 hasConcept C86803240 @default.
- W2365720654 hasConcept C96007430 @default.
- W2365720654 hasConceptScore W2365720654C10079403 @default.
- W2365720654 hasConceptScore W2365720654C104317684 @default.
- W2365720654 hasConceptScore W2365720654C147483822 @default.
- W2365720654 hasConceptScore W2365720654C153911025 @default.
- W2365720654 hasConceptScore W2365720654C159047783 @default.
- W2365720654 hasConceptScore W2365720654C159654299 @default.
- W2365720654 hasConceptScore W2365720654C16232356 @default.
- W2365720654 hasConceptScore W2365720654C186268636 @default.
- W2365720654 hasConceptScore W2365720654C2776441376 @default.
- W2365720654 hasConceptScore W2365720654C36394416 @default.
- W2365720654 hasConceptScore W2365720654C542903549 @default.
- W2365720654 hasConceptScore W2365720654C54355233 @default.
- W2365720654 hasConceptScore W2365720654C547475151 @default.
- W2365720654 hasConceptScore W2365720654C86803240 @default.
- W2365720654 hasConceptScore W2365720654C96007430 @default.
- W2365720654 hasIssue "2" @default.
- W2365720654 hasLocation W23657206541 @default.
- W2365720654 hasLocation W23657206542 @default.
- W2365720654 hasOpenAccess W2365720654 @default.
- W2365720654 hasPrimaryLocation W23657206541 @default.
- W2365720654 hasRelatedWork W165140220 @default.
- W2365720654 hasRelatedWork W2058813684 @default.
- W2365720654 hasRelatedWork W231659238 @default.
- W2365720654 hasRelatedWork W2355673107 @default.
- W2365720654 hasRelatedWork W2356249951 @default.
- W2365720654 hasRelatedWork W2356669090 @default.
- W2365720654 hasRelatedWork W2360487371 @default.
- W2365720654 hasRelatedWork W2365642106 @default.
- W2365720654 hasRelatedWork W2373220136 @default.
- W2365720654 hasRelatedWork W2374719815 @default.
- W2365720654 hasRelatedWork W2376753189 @default.
- W2365720654 hasRelatedWork W2377349240 @default.
- W2365720654 hasRelatedWork W2378381281 @default.
- W2365720654 hasRelatedWork W2381962498 @default.
- W2365720654 hasRelatedWork W2385345955 @default.
- W2365720654 hasRelatedWork W2386264952 @default.
- W2365720654 hasRelatedWork W2393292160 @default.
- W2365720654 hasRelatedWork W2393546180 @default.
- W2365720654 hasRelatedWork W3030083578 @default.
- W2365720654 hasRelatedWork W3030251100 @default.
- W2365720654 hasVolume "23" @default.
- W2365720654 isParatext "false" @default.
- W2365720654 isRetracted "false" @default.
- W2365720654 magId "2365720654" @default.
- W2365720654 workType "article" @default.