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- W2365759464 abstract "Using methods based upon the PCR, two DNA sequences which are identical to two JH genes(accession No.AY158087,AY149283) from one Holstein bovine genomic DNA have been cloned, the resultsdemonstrate that all the two JH genes(accession No.AY158087,AY149283) are present in the same Holsteinbovine genomic DNA. Two kinds of IgM cDNA was cloned from bovine spleen total RNA. Sequencing the cDNAclones show that the last exon of JH gene (accession No.AY149283) JH6 is a functional gene encoding part ofCDR3 and whole FR4 region of bovine IgM. The sequences of two IgM cDNA clones indicate each constantregion is identical to one of the two Cμgenes(accession No.AY230207,U63637), there are greater diversities inthe IgM CH1 domain than the other CH domains. PCR products of JH-Cμgene suggested that each Cμgene(accession No.AY230207,U63637) is in series with the same JH gene (accession No. AY149283) in the bovinegermline genes, they are all functional genes in the process of bovine IgM generation." @default.
- W2365759464 created "2016-06-24" @default.
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- W2365759464 date "2005-01-01" @default.
- W2365759464 modified "2023-09-25" @default.
- W2365759464 title "Cloning and Characterization of Two JH and CμIg Genes in Bovine Germline Gene" @default.
- W2365759464 hasPublicationYear "2005" @default.
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