Matches in SemOpenAlex for { <https://semopenalex.org/work/W2365760671> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2365760671 abstract "Objective To design and construct the fusion expression plasmid with TAT located at N terminal and C terminal of the fusion protein separately,to compare their discrepancy in penetrating activity,and then utilize TAT to transfect VP3 into cells to detect its proapoptotic activity. Methods Observe the differences in the efficiency of internalization between the two fusion proteins His-TAT-EGFP and His-EGFP-TAT with fluorescence microscopy and Western-blot. VP3 sequence was inserted into pET14b-EGFP-TAT,which possessed higher penetrating activity. The purified fusion protein His-VP3-EGFP-TAT was obtained and incubated with several kinds of cells including Hela,HEK293,3T3 and PC-3 to show its penetrating activity. Cell apoptosis was identified with the method of DAPI staining to observe the formation of nucleosome,and the VP3 fusion protein's proapoptotic activity was detected with MTT assay. Results In the protein concentration gradient experiment,fluorescence microscopy and Western-blot showed that His-EGFP-TAT had significantly higher penetrating activity than His-TAT-EGFP. When incubated with His-VP3-EGFP-TAT,strong fluorescence of particles might be found in all the different types of cells. After 48h incubation,the nucleosomes were found to form in experimental cells. MTT assay revealed that,compared to control group,the survival rates of Hela cells in the experimental group were reduced to 87.23±2.09%,53.01±1.79% and 42.52±1.90% at different fusion protein concentrations. The difference of survival rate of Hela cells between the two groups was statistically significant (P0.001). Conclusions TAT at N terminal of the fusion protein His-EGFP-TAT shows higher penetrating activity than that at C terminal of His-TAT-EGFP. The purified protein His-VP3-EGFP-TAT can internalize into different kinds of cells through their membranes and display efficient proapoptotic activity." @default.
- W2365760671 created "2016-06-24" @default.
- W2365760671 creator A5021753756 @default.
- W2365760671 date "2010-01-01" @default.
- W2365760671 modified "2023-09-26" @default.
- W2365760671 title "Optimization design of penetrating peptide TAT fusion expression vector and preliminary study on its targeting application" @default.
- W2365760671 hasPublicationYear "2010" @default.
- W2365760671 type Work @default.
- W2365760671 sameAs 2365760671 @default.
- W2365760671 citedByCount "0" @default.
- W2365760671 crossrefType "journal-article" @default.
- W2365760671 hasAuthorship W2365760671A5021753756 @default.
- W2365760671 hasConcept C104317684 @default.
- W2365760671 hasConcept C121332964 @default.
- W2365760671 hasConcept C123894998 @default.
- W2365760671 hasConcept C142613039 @default.
- W2365760671 hasConcept C1491633281 @default.
- W2365760671 hasConcept C153911025 @default.
- W2365760671 hasConcept C169274487 @default.
- W2365760671 hasConcept C185592680 @default.
- W2365760671 hasConcept C190283241 @default.
- W2365760671 hasConcept C2776415932 @default.
- W2365760671 hasConcept C2777366897 @default.
- W2365760671 hasConcept C2777527632 @default.
- W2365760671 hasConcept C2780783641 @default.
- W2365760671 hasConcept C33161422 @default.
- W2365760671 hasConcept C40767141 @default.
- W2365760671 hasConcept C54009773 @default.
- W2365760671 hasConcept C54355233 @default.
- W2365760671 hasConcept C55493867 @default.
- W2365760671 hasConcept C62520636 @default.
- W2365760671 hasConcept C81885089 @default.
- W2365760671 hasConcept C86803240 @default.
- W2365760671 hasConcept C91881484 @default.
- W2365760671 hasConceptScore W2365760671C104317684 @default.
- W2365760671 hasConceptScore W2365760671C121332964 @default.
- W2365760671 hasConceptScore W2365760671C123894998 @default.
- W2365760671 hasConceptScore W2365760671C142613039 @default.
- W2365760671 hasConceptScore W2365760671C1491633281 @default.
- W2365760671 hasConceptScore W2365760671C153911025 @default.
- W2365760671 hasConceptScore W2365760671C169274487 @default.
- W2365760671 hasConceptScore W2365760671C185592680 @default.
- W2365760671 hasConceptScore W2365760671C190283241 @default.
- W2365760671 hasConceptScore W2365760671C2776415932 @default.
- W2365760671 hasConceptScore W2365760671C2777366897 @default.
- W2365760671 hasConceptScore W2365760671C2777527632 @default.
- W2365760671 hasConceptScore W2365760671C2780783641 @default.
- W2365760671 hasConceptScore W2365760671C33161422 @default.
- W2365760671 hasConceptScore W2365760671C40767141 @default.
- W2365760671 hasConceptScore W2365760671C54009773 @default.
- W2365760671 hasConceptScore W2365760671C54355233 @default.
- W2365760671 hasConceptScore W2365760671C55493867 @default.
- W2365760671 hasConceptScore W2365760671C62520636 @default.
- W2365760671 hasConceptScore W2365760671C81885089 @default.
- W2365760671 hasConceptScore W2365760671C86803240 @default.
- W2365760671 hasConceptScore W2365760671C91881484 @default.
- W2365760671 hasLocation W23657606711 @default.
- W2365760671 hasOpenAccess W2365760671 @default.
- W2365760671 hasPrimaryLocation W23657606711 @default.
- W2365760671 hasRelatedWork W1533171028 @default.
- W2365760671 hasRelatedWork W1992976286 @default.
- W2365760671 hasRelatedWork W2041818425 @default.
- W2365760671 hasRelatedWork W2084266841 @default.
- W2365760671 hasRelatedWork W2333228856 @default.
- W2365760671 hasRelatedWork W2353500459 @default.
- W2365760671 hasRelatedWork W2354983219 @default.
- W2365760671 hasRelatedWork W2356833451 @default.
- W2365760671 hasRelatedWork W2357517417 @default.
- W2365760671 hasRelatedWork W2379387250 @default.
- W2365760671 hasRelatedWork W2381247604 @default.
- W2365760671 hasRelatedWork W2386363344 @default.
- W2365760671 hasRelatedWork W2405132662 @default.
- W2365760671 hasRelatedWork W2412236920 @default.
- W2365760671 hasRelatedWork W2771898152 @default.
- W2365760671 hasRelatedWork W2903513674 @default.
- W2365760671 hasRelatedWork W3029010752 @default.
- W2365760671 hasRelatedWork W3128997451 @default.
- W2365760671 hasRelatedWork W3029743572 @default.
- W2365760671 hasRelatedWork W3029791175 @default.
- W2365760671 isParatext "false" @default.
- W2365760671 isRetracted "false" @default.
- W2365760671 magId "2365760671" @default.
- W2365760671 workType "article" @default.