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- W2365763785 abstract "AIM To establish the determination method for analysing monosaccharide composition of polysaccharide from Juglans sigillata.METHODS The walnut shell polysaccharide was hydrolyzed by trifluoroacetic acid to give the hydrolysate containing monosaccharide which reacted with PMP to produce the PMP derivatives.The PMP derivatives were analyzed by HPLC.The concentration of gentiopicroside determined by RP-HPLC method was carried out on Agilent Venusil XBP-C18 column(4.6 mm×250 mm,5 μm),and the mobile phase was composed of acetonitrile-ammonium acetate buffer(pH 5.5)(19∶ 81).The flow rate was 1.0 mL/min,and the detection wavelength was set at 250 nm with column temperature of 30 ℃.RESULTS The polysaccharide from Juglans sigillata was broken into mannose,rhamnose,galactose,D-(+) galacturonic acid,glucose,D-glucuronic acid and D(-) arabinose(included four unidentifiable monosaccharide) with the linear ranges of 0.041 8-2.09 μg,0.045 9-2.295 μg,0.043 5-2.175 μg,0.042 7-2.135 μg,0.040 5-2.026 μg,0.043 9-2.195 μg and 0.047 5-2.375 μg,respectively,and with the average recoveries of 95.9%,103.1%,104.6%,97.5%,95.8%,87.5%,98.1%,respectively.The RSD,were less than 3.0%.The average content of highest among walnut shell extracts was D(-) arabinose.CONCLUSION This method can accurately determine the monosaccharide compositions and contents of polysaccharide from Juglans sigillata." @default.
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- W2365763785 date "2012-01-01" @default.
- W2365763785 modified "2023-10-15" @default.
- W2365763785 title "Monosaccharide composition of polysaccharide from Juglans sigillata D." @default.
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