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- W2365782143 abstract "Based on the mRNA sequence of human Suv39h2 gene,a porcine fragment of its cDNA sequence was obtained using in silico cloning method.By RT-PCR method,a porcine Suv39h2 cDNA fragment of 1 291 bp was amplified from muscle tissue and inserted into pGEX-KG plasmid expressing glutathione S-transferase(GST) fusion protein.After confirmation by the sequencing and restriction enzyme analysis,the recombinant plasimids were transformed into E.coli BL21 competent cells.The optimal IPTG induction conditions were determined as follows: induction time was 4 h and IPTG concentration was 0.7 mmol·L-1.The fusion protein was insoluble.Using Western blotting,we found a band with molecular weight of 66 kD,which indicated that porcine Suv39h2 was expressed in E.coli.This work laid a foundation for further work on functional study." @default.
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- W2365782143 date "2009-01-01" @default.
- W2365782143 modified "2023-09-24" @default.
- W2365782143 title "Cloning of Porcine Suv39h2 cDNA and Its Expression in E. coli" @default.
- W2365782143 hasPublicationYear "2009" @default.
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