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- W2365798553 abstract "Objective To study the effect of recombinant adeno-associated virus with T-bet gene(rAAV-T-bet) transfection on airway inflammation in mice infected with respiratory syncytial virus(RSV).Methods Forty Balb/c mice(aged 6-8 weeks) were randomly divided into 4 groups:control group(group A),RSV model group(group B),model/control recombinant adeno-associated virus carrying enhanced green fluorescent protein(rAAV-eGFP) intervention group(group C)and model/rAAV-T-bet virus intervention group(group D),10 mice in each group.Under intraperitoneal anesthesia,except group A,other groups were infected with nasal drip of 100 μL RSV which was harvested from Hep-2 cell culturer,rats in group A received Hep-2 cell culturer supernatant.Five days later,anesthetized mice intra-peritoneally once again,rats in group A and group B received nasal drip of 100 μL 293 cell culturer supernatant,rats in group C intranasal instillation of rAAV-eGFP virus and rats in group D intranasal instillation of rAAV-T-bet virus with equal volume as group A.The mice were killed 7 days later,bronchoalveolar lavage fluid(BALF)cell count and differential were studied.IL-4 and interferon-γ(IFN-γ) in BALF were determined.Pathologic changes in lung tissues were observed.Results 1.There were significant differences of the numbers of inflammation cells and the persentage of lymphocytes in BALF in group B[(43.91±10.41)×109L-1,(65.15±1.88)%,respectively]compared with those in group A[(14.52±3.57)×109L-1,(25.79±1.07)%,respectively].The numbers of inflammation cells and lymphocytes in BALF in group D[(24.84±4.35)×109L-1,(29.48±11.97)%,respectively]were also significantly different compared with those in group B and group C(Pa0.05).2.The IL-4 and IFN-γ levels in BALF in group B[(74.32±10.06) ng·L-1,(89.36±20.38) ng·L-1,respectively]were significantly different compared with those in group A[(6.01±1.02) ng·L-1,(177.32±18.16) ng·L-1,respectively](Pa0.05).The IL-4,IFN-γ levels in group D[(49.14±7.59) ng·L-1,(145.28±43.52) ng·L-1,respectively]were also different significantly compared with those in group B and group C(Pa0.05).3.There was no obvious infiltration of inflammatory cells in the lung tissue in group A.However,there were a great number of inflammatory cells in the interstitial and peribronchovascular regions in group B and group C.Group D exhibited reduced epithelial damage.Conclusions rAAV-T-bet gene transfection is effective in regulating the abnormal level of cytokines(IL-4 and IFN-γ)in BALF and inhibiting airway inflammation of RSV infection in mice." @default.
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- W2365798553 date "2011-01-01" @default.
- W2365798553 modified "2023-09-27" @default.
- W2365798553 title "Effect of Recombinant Adeno Associated Virus Carrying T-bet Gene Transfection on Airway Inflammation of Mice Infected with Respiratory Syncytial Virus" @default.
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