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- W2365811372 abstract "This study aims to prepare and identify monoclonal antibodies against Borna disease virus(BDV) nucleoprotein.Balb/c mice were immunized with recombinant BDV nucleoprotein and the spleen cells of the immunized mice were fused with the SP2/0 myeloma cells.The positive clones were selected and subcloned 3 times.One hybridoma cell line secreting specific McAb against BDV nucleoprotein was obtained which named 2F6E8,and the McAb was belonged to IgG2a.ELISA showed that the titers of the ascites was 1∶32 000;Western blotting and IFA experiments illustrated that the monoclonal antibody could specifically bind to BDV nucleoprotein.This polyclonal antibody may provide the foundation for the further studies on BDV serum immunology detection methods." @default.
- W2365811372 created "2016-06-24" @default.
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- W2365811372 date "2012-01-01" @default.
- W2365811372 modified "2023-09-23" @default.
- W2365811372 title "Preparation and identification of monoclonal antibody against nucleoprotein of Borna disease virus" @default.
- W2365811372 hasPublicationYear "2012" @default.
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