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- W2365822879 abstract "AimTo observe the effect of human granzyme B fusion protein on the HeLa cell line. MethodsHuman granzyme B whole cDNA was obtained by RT-PCR. N terminus of active granzyme B gene was fused with the transmembrane domain in PE sequence. The resulting fusion protein gene was cloned into GFP co-expression vector pIRES2-EGFP and transfected into HeLa cells. Expression of target protein was detected by immunochemical staining with antigranzyme B PcAb. Changes of the morphology and structure of HeLa cells subjected to transfection of target gene were observed under fluorescent and electronic microscopes.ResultsWild type human granzyme B cDNA and its fusion protein gene were cloned and co-expression vector EGFP-P280LG was constructed Successfully. The target protein was detected in the cytoplasm of transfected HeLa cells. Some transfected cells appeared as volume extension and often was in the wake of multiple nuclei, while others displayed typical features of apoptosis.ConclusionEctopic expression of granzyme B fusion protein in HeLa cells leads to morphological and structural changes including appearance of giant cells with multiple nuclei or apoptotic cells with karyopyknosis." @default.
- W2365822879 created "2016-06-24" @default.
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- W2365822879 date "2002-01-01" @default.
- W2365822879 modified "2023-10-18" @default.
- W2365822879 title "The effect of human granzyme B -PE domain IIfusion protein on transfected HeLa cells" @default.
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