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- W2365842783 abstract "Objective To study the CEA expression and immune responses elicited by carcinoembryonic antigen DNA vaccine with different Promoters, pCEA and pLCEASN.Methods Twenty mice, who were intramuscularly injected with 0.75% bupivacaine 7 days ago, were randomly divided into 4 groups of 5 animals, who received intramuscular injection of100μg pCEA, pLCEASN,pcDNA3 or pLXSN, every other week for five weeks,respectively. At 7 days after the last injection, the sera were harvested for detection of anti - CEA, then all mice were sacrificed and the tongue were removed for detecting CEA with radioimmunoassay. Results After immunization with pLCEASN and pCEA,the CEA contents in the tongne muscle were (1.85±0.11) ng/mg and (2.48±0.09) ng/mg respectively,between which there was significant difference ( P 0.001), and the titre of anti - CEA ( OD490) in the sera (1:200) were 0.42±0.04 and 0.59±0.06 respectively, between which there was significant difference ( P 0.001). Conclusion The promoter of careinoembryonic antigen DNA vaccine has an important effect on the expression of CEA and humoral immune response." @default.
- W2365842783 created "2016-06-24" @default.
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- W2365842783 date "2005-01-01" @default.
- W2365842783 modified "2023-09-23" @default.
- W2365842783 title "Effect of Different Promoters on CEA Expression and Immune Responses Elicited by Carcinoembryonic Antigen DNA Vaccine in Mice" @default.
- W2365842783 hasPublicationYear "2005" @default.
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