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- W2365852471 abstract "E2 gene was successfully amplified by PCR using genome DNA of the wild yak as template.In order to study the antigenicity of E2 protein,we inserted E2 gene into pET-32a vector and reconstructed plasmid pET-32a-E2 and then transformed them into BL21(DE3) host bacterium,using IPTG to induce them to express in the end.The result showed that a 58 kD fusion protein which was detected by SDS-PAGE was expressed efficiently by pET-32a-E2 in the BL21(DE3) host bacterium and the size of expressed protein was consistent with our anticipation.Western-blotting results showed that E2 protein has a good antigenicity.The research is important for the future study of the function of E2 protein." @default.
- W2365852471 created "2016-06-24" @default.
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- W2365852471 date "2011-01-01" @default.
- W2365852471 modified "2023-09-24" @default.
- W2365852471 title "Cloning and Prokaryotic Expression of E2 Genes of Bovine Viral DiarrheaVirus Strain from Yak" @default.
- W2365852471 hasPublicationYear "2011" @default.
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