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- W2365854986 abstract "Liver receptor homolog-1( LRH-1) is an orphan member of the Ftz-F1 subfamily of nuclear receptors. LRH-1 is highly expressed in ovary and so it is thought to be involved in the regulation of steroidogenesis in ovary. Here we isolated LRH-1 cDNA and inactive LRH-1′ cDNA in yellow catfish( Pelteobagrus fulvidraco) using RT-PCR and RACE method. The yellow catfish LRH-1 cDNA( 1 945 bp) had a 207 bp 5′-untranslated region( UTR),a 238 bp 3′ UTR and a 1 500 bp open reading frame,which encoded a 500-amino-acid residues protein with deduced molecular weight of 57.19 kD. However,LRH-1′ was 1 663 bp with 283 bp 5′UTR,330 bp 3′ UTR and a 1 050 bp open reading frame,which encoded a 350-amino-acid residues protein with deduced molecular weight of 39.7 kD. Compared with LRH-1,LRH-1′ were absent of zinc-fingers and AF-2 motif. The alignment results of LRH-1’ s amino acid sequences indicated the similarity was from 79% to 85% between yellow catfish LRH-1 and other vertebrate animals’ counterparts,and was from 59% to 66% between yellow catfish LRH-1 and other animals’ steroidogenic factor-1( SF-1),another member of Ftz-F1 subfamily. Using Clustal W,we constructed the phylogenetic tree of the Ftz-F1 which showed two main branches. All teleost SF-1 clustered into one branches,and the other branches bifurcated into two clear sub ones,one of which contained other animals’ SF-1 and the other contained two smaller groups of all teleost’ s LRH-1 and other animals’ LRH-1,rseparately. Real-time RT-PCR with β-actin as internal control was used to explore the expression levels of LRH-1 in brain( including forebrain,hypothalamus,pituitary),liver,kidney and gonad. The results indicated LRH-1 expressed in brain,liver, kidney,and gonad with relatively high level in male and female fish liver. The expression levels in female liver and ovary were significantly higher than in male fish liver and testis,respectively. In ovary,the expression level of LRH-1 varied with the developmental phases. There were two highest expression levels in overy of phase III and V. After spawning or degeneration of ovary,the LRH-1 expression level decreased sharply. And after 12 h of LRH-A intramuscular injection, the expression level of LRH-1 significantly decreased in ovary. However,it significantly increased in liver and kidney." @default.
- W2365854986 created "2016-06-24" @default.
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- W2365854986 date "2009-01-01" @default.
- W2365854986 modified "2023-09-24" @default.
- W2365854986 title "Isolation and expression pattern of LRH-1 in yellow catfish (Pelteobagrus fulvidraco)" @default.
- W2365854986 hasPublicationYear "2009" @default.
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