Matches in SemOpenAlex for { <https://semopenalex.org/work/W2365866486> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2365866486 abstract "Human FL(flt3 ligand) is expressed in E.coli for clinical application and functional study of hFL. An artificial DNA fragment encoding FL outmemberance region was synthesized by using favored genetic codons in E.coli. The sequence of hFL DNA fragment prepared by PCR is confirmed to be correct. Recombinant expressing vector pET30a-Trx-hFL was transformated into E.coli BL21(DE3). The yield of Trx-hFL is over 60% of total cellular protein and expressed as inclusion bodies. Trx-hFL was purified with anion-exchange and gel filtration. The fusion protein was cleavaged by FXa with cleavage efficiency over 80%. rhFL was purified by affinity chromatography. The purified protein was identified by Western blot. The biological activity of rhFL in combination with GM-CSF and TNFα was examined by stimulating proliferation of dendritic cells from cord blood. The purified rhFL has biological activity in stimulating dendritic cell proliferation in combination with GM-CSF and TNFα in vitro." @default.
- W2365866486 created "2016-06-24" @default.
- W2365866486 creator A5003275608 @default.
- W2365866486 date "2002-01-01" @default.
- W2365866486 modified "2023-09-23" @default.
- W2365866486 title "Expression of human FL in E.coli and the purification as well as bioassay of its expression product" @default.
- W2365866486 hasPublicationYear "2002" @default.
- W2365866486 type Work @default.
- W2365866486 sameAs 2365866486 @default.
- W2365866486 citedByCount "0" @default.
- W2365866486 crossrefType "journal-article" @default.
- W2365866486 hasAuthorship W2365866486A5003275608 @default.
- W2365866486 hasConcept C104317684 @default.
- W2365866486 hasConcept C116073593 @default.
- W2365866486 hasConcept C123894998 @default.
- W2365866486 hasConcept C153911025 @default.
- W2365866486 hasConcept C179247698 @default.
- W2365866486 hasConcept C181199279 @default.
- W2365866486 hasConcept C202751555 @default.
- W2365866486 hasConcept C203750385 @default.
- W2365866486 hasConcept C2776415932 @default.
- W2365866486 hasConcept C33161422 @default.
- W2365866486 hasConcept C40767141 @default.
- W2365866486 hasConcept C547475151 @default.
- W2365866486 hasConcept C55493867 @default.
- W2365866486 hasConcept C86803240 @default.
- W2365866486 hasConceptScore W2365866486C104317684 @default.
- W2365866486 hasConceptScore W2365866486C116073593 @default.
- W2365866486 hasConceptScore W2365866486C123894998 @default.
- W2365866486 hasConceptScore W2365866486C153911025 @default.
- W2365866486 hasConceptScore W2365866486C179247698 @default.
- W2365866486 hasConceptScore W2365866486C181199279 @default.
- W2365866486 hasConceptScore W2365866486C202751555 @default.
- W2365866486 hasConceptScore W2365866486C203750385 @default.
- W2365866486 hasConceptScore W2365866486C2776415932 @default.
- W2365866486 hasConceptScore W2365866486C33161422 @default.
- W2365866486 hasConceptScore W2365866486C40767141 @default.
- W2365866486 hasConceptScore W2365866486C547475151 @default.
- W2365866486 hasConceptScore W2365866486C55493867 @default.
- W2365866486 hasConceptScore W2365866486C86803240 @default.
- W2365866486 hasLocation W23658664861 @default.
- W2365866486 hasOpenAccess W2365866486 @default.
- W2365866486 hasPrimaryLocation W23658664861 @default.
- W2365866486 hasRelatedWork W126763208 @default.
- W2365866486 hasRelatedWork W1990880275 @default.
- W2365866486 hasRelatedWork W2071391374 @default.
- W2365866486 hasRelatedWork W2357675544 @default.
- W2365866486 hasRelatedWork W2358498657 @default.
- W2365866486 hasRelatedWork W2362236774 @default.
- W2365866486 hasRelatedWork W2367044468 @default.
- W2365866486 hasRelatedWork W2368701960 @default.
- W2365866486 hasRelatedWork W2369877836 @default.
- W2365866486 hasRelatedWork W2373166638 @default.
- W2365866486 hasRelatedWork W2374068364 @default.
- W2365866486 hasRelatedWork W2374784578 @default.
- W2365866486 hasRelatedWork W2379488569 @default.
- W2365866486 hasRelatedWork W2385873988 @default.
- W2365866486 hasRelatedWork W2386275730 @default.
- W2365866486 hasRelatedWork W2388094403 @default.
- W2365866486 hasRelatedWork W2392346921 @default.
- W2365866486 hasRelatedWork W2397030661 @default.
- W2365866486 hasRelatedWork W3143208977 @default.
- W2365866486 hasRelatedWork W3149709944 @default.
- W2365866486 isParatext "false" @default.
- W2365866486 isRetracted "false" @default.
- W2365866486 magId "2365866486" @default.
- W2365866486 workType "article" @default.