Matches in SemOpenAlex for { <https://semopenalex.org/work/W2365908043> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2365908043 endingPage "604" @default.
- W2365908043 startingPage "600" @default.
- W2365908043 abstract "Objective To study the resistant phenotype of a clinical strain of Escherichia coli and to explore the effect of its attenuator mutation on AmpC expression. Methods A clinical strain of Escherichia coli 20022 (ECO20022) resistant to cefoxitin was isolated clinically. The phenotype was examined by three-dimensional methods, isoelectric focusing (IEF), and microdilution method. The regulator genes of ECO20022 were amplified and sequenced, and the difference between them was analyzed by BLAST method. Then the regulator genes were cloned into pCAT3-basic vector (a promoterless reporter gene vector). Microdilution method was used to detect the minimal inhibitory concentration (MIC) of chloramphenicol and ampicillin to this strain with E. coli ATCC25922 as quality control bacterium. ELISA was used to detect the content of chloramphenicol acetyl transferase (CAT). Results Compared to the standard E. coli K-12, there were four base substitutions, i.e., 22C-T, 26, 27TA-GT, and 32G-A in the attenuator region of ECO20022. Three-dimensional method showed that this strain was high AmpC-producing. IEF found that it produced three beta-lactamases with the values of PI of 5.4, 8.2, and 9.0 respectively. The beta-lactamase with the PI of 9.0 could be inhibited by cloxacillin but not by clavulanate. The strain was resistant to not only most of third generation cephalosporins, but also to cefepime; however it was still susceptible to carbapenem. The secondary structure of the attenuator RNA of ECO20022 was different from the traditional structure of E. coli K-12. The regulator gene was successfully cloned into pCAT3-basic vector and direct and indirect tests indicated that this regulator gene enhanced the CAT expressing level as much as 10 times that of Escherichia coli K-12. Conclusion AmpC attenuator mutation leads to high AmpC expression in Escherichia coli, resulting in a significant rise of resistance level to beta-lactamase and a great menace to clinical antibiotic therapy." @default.
- W2365908043 created "2016-06-24" @default.
- W2365908043 creator A5011268957 @default.
- W2365908043 creator A5035280226 @default.
- W2365908043 creator A5038050579 @default.
- W2365908043 creator A5038862882 @default.
- W2365908043 creator A5041674190 @default.
- W2365908043 creator A5059590602 @default.
- W2365908043 creator A5064633137 @default.
- W2365908043 date "2006-03-07" @default.
- W2365908043 modified "2023-09-27" @default.
- W2365908043 title "[Expression of high-level cephalosporinase due to mutation in the AmpC attenuator of a clinical Escherichia coli strain]." @default.
- W2365908043 doi "https://doi.org/10.3760/j:issn:0376-2491.2006.09.009" @default.
- W2365908043 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16681904" @default.
- W2365908043 hasPublicationYear "2006" @default.
- W2365908043 type Work @default.
- W2365908043 sameAs 2365908043 @default.
- W2365908043 citedByCount "0" @default.
- W2365908043 crossrefType "journal-article" @default.
- W2365908043 hasAuthorship W2365908043A5011268957 @default.
- W2365908043 hasAuthorship W2365908043A5035280226 @default.
- W2365908043 hasAuthorship W2365908043A5038050579 @default.
- W2365908043 hasAuthorship W2365908043A5038862882 @default.
- W2365908043 hasAuthorship W2365908043A5041674190 @default.
- W2365908043 hasAuthorship W2365908043A5059590602 @default.
- W2365908043 hasAuthorship W2365908043A5064633137 @default.
- W2365908043 hasConcept C104317684 @default.
- W2365908043 hasConcept C153911025 @default.
- W2365908043 hasConcept C2778473333 @default.
- W2365908043 hasConcept C2780920689 @default.
- W2365908043 hasConcept C501593827 @default.
- W2365908043 hasConcept C54355233 @default.
- W2365908043 hasConcept C547475151 @default.
- W2365908043 hasConcept C86803240 @default.
- W2365908043 hasConcept C89423630 @default.
- W2365908043 hasConceptScore W2365908043C104317684 @default.
- W2365908043 hasConceptScore W2365908043C153911025 @default.
- W2365908043 hasConceptScore W2365908043C2778473333 @default.
- W2365908043 hasConceptScore W2365908043C2780920689 @default.
- W2365908043 hasConceptScore W2365908043C501593827 @default.
- W2365908043 hasConceptScore W2365908043C54355233 @default.
- W2365908043 hasConceptScore W2365908043C547475151 @default.
- W2365908043 hasConceptScore W2365908043C86803240 @default.
- W2365908043 hasConceptScore W2365908043C89423630 @default.
- W2365908043 hasIssue "9" @default.
- W2365908043 hasLocation W23659080431 @default.
- W2365908043 hasLocation W23659080432 @default.
- W2365908043 hasOpenAccess W2365908043 @default.
- W2365908043 hasPrimaryLocation W23659080431 @default.
- W2365908043 hasRelatedWork W1948480583 @default.
- W2365908043 hasRelatedWork W1996960109 @default.
- W2365908043 hasRelatedWork W2031141462 @default.
- W2365908043 hasRelatedWork W2032183556 @default.
- W2365908043 hasRelatedWork W2049689083 @default.
- W2365908043 hasRelatedWork W2058935505 @default.
- W2365908043 hasRelatedWork W2077280669 @default.
- W2365908043 hasRelatedWork W2086784228 @default.
- W2365908043 hasRelatedWork W2097181820 @default.
- W2365908043 hasRelatedWork W2125603305 @default.
- W2365908043 hasRelatedWork W2147721260 @default.
- W2365908043 hasRelatedWork W2161736374 @default.
- W2365908043 hasRelatedWork W2165449795 @default.
- W2365908043 hasRelatedWork W2191001091 @default.
- W2365908043 hasRelatedWork W2409029218 @default.
- W2365908043 hasRelatedWork W2472709216 @default.
- W2365908043 hasRelatedWork W2563364812 @default.
- W2365908043 hasRelatedWork W2782587362 @default.
- W2365908043 hasRelatedWork W2803813675 @default.
- W2365908043 hasRelatedWork W2979844240 @default.
- W2365908043 hasVolume "86" @default.
- W2365908043 isParatext "false" @default.
- W2365908043 isRetracted "false" @default.
- W2365908043 magId "2365908043" @default.
- W2365908043 workType "article" @default.