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- W2365913904 abstract "Objective To compare the influences of two kinds of nucleic acid extraction method for detecting hepatitis C virus(HCV)RNA viral load by the real-time fluorescenct quantitative(FQ)PCR.Methods 89 inpatients with chronic hepatitis C(CHC)and anti-HCV positive in the Therapy Center for Liver Diseases of our hospital from October 2012 to June 2013 were selected and HCVRNA was extracted from the serum specimens by using the magnetic beads and Trizol methods.FQ-PCR was adopted for detecting serum HCV RNA.The detected results were performed the comparative analysis.The statistic processing adopted theχ2 test and t test.Results The positive rate of serum HCV RNA extracted by the magnetic beads and Trizol methods were 73.0%(65/89)and 65.1%(58/89)respectively,and the difference between the two methods showed the statistical significance(χ2=3.76,P0.05).By converting the concentration results detected by these two methods into the log values,there was no statistically significant difference between the two methods(t=0.32,P0.05).Conclusion The two kinds of method can obtain the higher recovery rate with higher sensitivity and higher specificity.The magnetic beads method for extracting HCV RNA could be used in the clinical diagnosis and therapeutic observation in the HCV infected persons." @default.
- W2365913904 created "2016-06-24" @default.
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- W2365913904 date "2015-01-01" @default.
- W2365913904 modified "2023-09-23" @default.
- W2365913904 title "Comparison of effects between two kinds of nucleic acid extraction method for detecting HCV RNA and their application evaluation" @default.
- W2365913904 hasPublicationYear "2015" @default.
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