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- W2365922497 abstract "A purified plasma membrane fraction was isolated using differential centrifugation combined with aqueous two-phase partitioning from wheat Feekes 8.5 flag leaves.The lysis buffer was optimized for two-dimensional electrophoresis(2-DE) analysis,and the conditions of different gel concentrations and sample loading were compared.The results showed that high purity plasma membrane of 89.2% was obtained at 6.4% PEG 3350/Dextran T-500(W/W) in our two-phase system.The plasma membrane proteins were extracted by the TCA/acetone method,loaded in the amount of 900 μg and resolved by a 17 cm pH 4 ~ 7 IPG and 12% SDS-PAGE gel,then visualized by CBB G-250 staining.A total of 173 protein spots were detected by the PDQuest 2DE 8.0.1 software.The optimization of 2-DE protocol allowed a suitable condition for proteomic analysis of plasma membrane from wheat flag leaves." @default.
- W2365922497 created "2016-06-24" @default.
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- W2365922497 date "2013-01-01" @default.
- W2365922497 modified "2023-09-25" @default.
- W2365922497 title "Preparation of Plasma Membrane with High Purity from Wheat Flag Leaves for 2-DE Proteomics" @default.
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