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- W2365932455 abstract "Objective To study the frequency of distribution and molecular genetic background of Jk(a-b-)donors in Guangzhou.Methods Blood samples were collected from 50 000donors in Guangzhou Blood Center.Urea lyses method was used to screening the Jk(a-b-)individuals.Then the phenotype was confirmed by the standard serological methods.DNAs were extracted from venous blood.PCR method was adopted to amplify the coding exons of Jk gene and then PCR products were sequenced directly.Results Ten donors with Jk(a-b-)phenotype were screened from 50 000 donors and the frequency of Jk(a-b-)was 0.02%.Eight DNA samples were available to sequencing Jk gene.Two genotyping results(homozygous IVS5-1GA,n=6;heterozygous IVS5-1GA/heterozygous 896GA,n=2)were identified.Conclusion The frequency of Jk(a-b-)in Guangzhou blood donors is higher than the reported from populations in Northern regions of China.The IVS5-1GA mutation,distributed in Polynesians with high frequency,is also the most common variations identified in Guangzhou populations." @default.
- W2365932455 created "2016-06-24" @default.
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- W2365932455 date "2012-01-01" @default.
- W2365932455 modified "2023-09-25" @default.
- W2365932455 title "Study on the frequency of distribution and molecular genetic background of Jk(a-b-)donors in Guangzhou" @default.
- W2365932455 hasPublicationYear "2012" @default.
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