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- W2365934279 abstract "AIM: To construct an adipose differentiation-related protein(ADRP) eukaryotic expression vector and to explore the effect of ADRP on apoptosis of H9c2 cells induced by palmitic acid(PA).METHODS: The ADRP gene obtained by the method of RT-PCR was cloned into pEGFP-C1 plasmid.The recombinant plasmid was transformed into E.coli DH5α for amplification.The recombinant plasmid was extracted from E.coli DH5α and transfected into H9c2 cells by LipofectamineTM2000.The stable transformants were selected by G418 screening.Expression of green fluorescent protein was observed under fluorescence microscope and the ADRP expression was identified by RT-qPCR and Western blotting analysis.The effect of PA on the proliferation of H9c2 cells was detected by MTT assay.The apoptotic percentage of H9c2 cells caused by PA was determined by flow cytometry.RESULTS: The eukaryotic expression vector pEGFP-C1-ADRP was successfully constructed.Green fluorescent was observed in the cells transfected with pEGFP-C1 or pEGFP-C1-ADRP under fluorescence microscope.RT-qPCR and Western blotting analysis showed that recombinant cells exhibited high mRNA and protein levels of ADRP.After treated with PA at different concentrations,the apoptosis rates and the proliferation inhibition of recombinant cells were both lower than those of the other two cells.CONCLUSION: The transfected H9c2 cells with stable ADRP expression were successfully established.The over-expression of ADRP prevents the cells from apoptosis and inhibition of proliferation caused by PA,indicating that ADRP plays a protective role in H9c2 cells." @default.
- W2365934279 created "2016-06-24" @default.
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- W2365934279 date "2011-01-01" @default.
- W2365934279 modified "2023-09-23" @default.
- W2365934279 title "Effect of adipose differentiation-related protein on proliferation and apoptosis in H9c2 cells" @default.
- W2365934279 hasPublicationYear "2011" @default.
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