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- W2365937564 abstract "Aim: To investigate the details of NK cells activation by mitogen in vitro with CD69 expression as activation marker for the application of these cells clinically. Methods: After separating the PBMCs from adult human peripheral blood by Ficoll-Hypaque centrifugation, the PBMCs culture procedure was applied to activate NK cells in vitro . These cells were activated by PHA(20 μg/mL)and PDB(10 -7 mol/L)separately. The expression rates of CD69 of 0, 2, 12, 24 h-cultured NK cells were estimated by flow cytometry following two-color immunofluorescent staining. Results: The expression rates of CD69 after 2 hours stimulation in response to PHA and PDB were (40.90±5.17)% and (62.82±7 69)%, and those after 24 hours were (84.96±9.24)% and (91.85±2.94)% respectively. The rates in response to PDB were higher than those of PHA at all time points. Conclusion: PHA、PDB may upregulate rapidly the expression of CD69 on NK cells in vitro and the effect of PDB(10 -7 mol/L)was stronger than that of PHA(20 μg/mL). [" @default.
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- W2365937564 date "2001-01-01" @default.
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- W2365937564 title "Study on the expression of CD69 on NK cells activated in vitro by mitogen from adult human peripheral blood" @default.
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