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- W2365961596 abstract "Objective:To investigate whether aspirin had influences on endothelial progenitor cells(EPCs).Methods:Total mononuclear cells(MNCs) were isolated from peripheral blood by ficoll density gradient centrifugation,and then the cells were plated on fibronectin-coated culture dishs.After 7 days cultured,attached cells were stimulated with aspirin(to make a series of final concentrations:1,2,5,10mmol/L)for 3,6,12,24h.EPCs were characterized as adherent cells double positive for DiLDL-uptake and lectin binding by direct fluorescent staining.EPCs proliferation,migration were assayed with MTT assay and modified Boyden chamber assay,respectively.EPCs adhesion assay was performed by replating those on fibronectin-coated dishes,and then adherent cells were counted.In vitro vasculogenesis activity was assayed by in vitro vasculogenesis kit.iNOS was assayed by western blot.Results:Incubation of isolated human MNCs with aspirin decreased the number of EPCs,aspirin also decreased EPCs proliferative,migratory,adhesive,in vitro vasculogenesis capacity and iNOS in a concentration and time dependent manner.Conclusion:Aspirin decrease the number,proliferative,migratory,adhesive and in vitro vasculogenesis capacity of EPCs,aspirin also decrease iNOS in EPCs." @default.
- W2365961596 created "2016-06-24" @default.
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- W2365961596 date "2007-01-01" @default.
- W2365961596 modified "2023-09-22" @default.
- W2365961596 title "Effects of aspirin on endothelial progenitor cell function and its induced nitricoxide synthase" @default.
- W2365961596 hasPublicationYear "2007" @default.
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