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- W2365973967 abstract "Aim: To clone and analyze sequences of the 5' upstream regions of the duplicated carbonic anhydrase (DCA1) and the carbonic anhydrase (CA1) from Dunaliella salina.Methods: The genomic DNA from Dunaliella salina was digested with Dra I,EcoR V,Pvu II and Stu I,respectively.A GenomeWalker Adaptor was ligated to the ends of the digested DNA fragments.Construction of GenomeWalker Libraries (GWL1,GWL2,GWL3 and GWL4) was finished.The 5' upstream regions of DCA1 and CA1 genes from Dunaliella salina GenomeWalker Libraries were amplified by nested PCR method.The DNA sequence was determined by dideoxy-mediated chain termination. Results: Single major PCR products of 1.3 kb and 4.5 kb fragments from the GWL1 and GWL3 in the DCA1 gene, and 1.7kb and 2.5kb fragments from the GWL2 and GWL4 in the CA1 gene were generated,respectively.The two specific fregments,1.3 kb and 1.7 kb,were cloned and sequenced.The partial sequences of 3' end in the 1.3 kb and 1.7 kb fragments were completely consistent with the partial sequences of 5' end of the DCA1 and CA1 gene cDNA, respectively.There were several conserved promoter motifs such as TATA-box,CAAT-box, and the tandem GT sequence in the sequences. Conclusion: The 5' upstream sequences obtained from the DCA1 and CA1 genes might be two novel promoters of carbonic anhydrase from Dunaliella salina,which were found in unknown genomic DNA sequences adjacent to a known sequence such as cDNA or other domain of regulation,by use of the GenomeWalker DNA walking." @default.
- W2365973967 created "2016-06-24" @default.
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- W2365973967 date "2004-01-01" @default.
- W2365973967 modified "2023-09-27" @default.
- W2365973967 title "Cloning and sequencing of the 5' upstream of the two carbonic anhydrase genes from Dunaliella salina" @default.
- W2365973967 hasPublicationYear "2004" @default.
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