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• W2365996817 abstract "This paper studies the effects of different electric field strength and treatment time on the inactivation of Ecoli suspended in sodium chloride(electric conductivity δ=340μS/cm,30°C) by a continuous high intensity pulsed electric field(PEF) treatment system.The plate counting method is used to determine the viability of the cells.The results show that the survival of Ecoli decreases with increasing the electric field strength and treatment time,and E.coli is inactivated by 30 kV/cm,266 ms treatment.The subsequent leakage of protein and nucleic acid of Ecoli due to the PEF processing is investigated by using the ultraviolet spectrophotometric method.And the relative amounts of the leaked protein and nucleic acid from Ecoli increase after the PEF treatment,and increase with increasing the electric field strength and treatment time,showing that PEF treatment increases the membrane permeabilization of Ecoli.ERIC(Enterbacteria Repetitive Intergenic Consensus)-PCR method is used to determine the inactivation of PEF on Ecoli.ERIC-PCR fingerprinting demonstrated that DNA of the treated Ecoli is injured and DNA degradation appeares when the electric field strength is up to 20kV/cm for 539ms or more severe PEF treatment(30kV/cm for 266ms;35kV/cm for 266ms;50kV/cm for177ms),and macromolecule DNA is degraded into small molecule fragments.The results indicate the increased membrane permeability and DAN degradation may resulted in the lethal of Ecoli." @default.
• W2365996817 created "2016-06-24" @default.
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• W2365996817 date "2007-01-01" @default.
• W2365996817 modified "2023-09-28" @default.
• W2365996817 title "Effect of High Intensity Pulsed Electric Field on the Membrane Permeability and DNA of E coli" @default.
• W2365996817 hasPublicationYear "2007" @default.
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