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- W2366021164 abstract "With the technology of PCR,K88ac gene and ST1-LTB fusion genes were amplified respectively from the plasmid of Escherichia coli C83902 and the recombinant plasmid pXSLT1.The recombinant plasmid pET28-KSL containing fusion gene K88ac-ST1-LTB was obtained and transformed into Escherichia coli BL21(DE3).The recombinant plasmid pET28-KSL contained the fusion gene K88ac-ST1-LTB which had correct sequence and ORF by identification of endonuclease-digesting and sequence analysis.The fusion proteins K88ac-ST1-LTB were expressed in the recombinant strain BL21(DE3)(pET28-KSL).By ELISA method,the expressed protein could be recognized by MAb of ST1,LTB and K88ac antibodies.The result indicated that the fusion gene K88ac-ST1-LTB of enterotoxigenic Escherichia coli was successfully constructed and expressed in Escherichia coli." @default.
- W2366021164 created "2016-06-24" @default.
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- W2366021164 date "2009-01-01" @default.
- W2366021164 modified "2023-09-23" @default.
- W2366021164 title "Gene Fusion of Enterotoxigenic Escherichia coli K88ac Gene,ST_1 Gene and LT_B Gene" @default.
- W2366021164 hasPublicationYear "2009" @default.
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