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- W2366028021 abstract "Real-time PCR was used for detection of Leptosphaeria maculans,the causative agent of oilseed phoma stem canker.Specific primers Lmb3/R2 and a TaqMan-MGB probe(Probe-M) specific to L.maculans were designed from the differentiation of ITS sequences between L.maculans and related species in the GenBank database.The results showed that all 22 isolates of L.maculans from oilseed samples from Austra-lia,Ukraine and Canada could get positive amplification with the limit of 4 pg DNA,and negative results for 30 isolates of L.biglobosa and the other 6 isolates of related species from oilseed samples.Real-time PCR could be especially useful for mass screening of oilseed sample and diagnosis of suspected isolate." @default.
- W2366028021 created "2016-06-24" @default.
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- W2366028021 date "2011-01-01" @default.
- W2366028021 modified "2023-09-23" @default.
- W2366028021 title "Detection of Leptosphaeria maculans by real-time PCR" @default.
- W2366028021 hasPublicationYear "2011" @default.
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