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- W2366036339 abstract "Objective:An Survivin antisence RNA/HSP70 double-gene expression vector was constructed to provide important experimental material for the further research,which was used to transfect the tumor cells,inhibit proliferation of tumor cells,induce apoptosis of tumor cells and stimulate the specific anti-tumor immune response effectively.Methods:A cDNA fragment of survivin obtained by RT-PCR was inserted into a plasmid vector named pIRES2-DsRed2 in the reverse direction.Survivin antisense RNA was confirmed using restriction enzyme digesion and DNA sequencing.A HSP70 cDNA fragment which was obtained from the HepG2 cell using RT-PCR was inserted into pPIRES-DeRed2.Results:It was verified that the Survivin antisense RNA expression vector had been successfully constructed by restriction enzyme digestion and DNA sequencing.By colony PCR,reseriction enzyme digestion and DNA sequencing,HSP70 expression vector had been proven to be successful constructed.Conclusions:It indicate that an survivin antisense RNA/HSP70 double-gene vector is successfully estabilished,which laid an experimental foundation for further study." @default.
- W2366036339 created "2016-06-24" @default.
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- W2366036339 date "2007-01-01" @default.
- W2366036339 modified "2023-09-23" @default.
- W2366036339 title "Construction and Indentification of Survivin Antisense RNA/HSP70 Double-Gene Expression Vector" @default.
- W2366036339 hasPublicationYear "2007" @default.
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