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- W2366059897 abstract "The polymorphisms of three species of nematodes from different sources, Moloidogyne javanica, Enterobius species and Ancylostoma caninum,were analyzed by means of RFLP in order to establish the basis for the further development of molecular diagnosis of nematodes from soil. In the present study,we used the relatively conserved region,the internal transcribed spacer(ITS) including partial sequnces of 5.8S, ITS2 and 28S and the genome DNA of these 3 different species of nematode as template to design the specific primers for the amplification of the ITS2 sequence. Six restrictive endonucleases,HindIII, EcoRI, EcoRV, PstI, KpnI and SalI, were used for the digestion of PCR products of ITS2. The experimental results showed that the sizes of the PCR products of these 3 species of nematodes were 1000, 1800, 1800 bps respectively. Twelve fragments were obtained after digestion of ITS2 with these restrictive enzymes in which two pairs of them, HindIII/EcoRI, PstI/EcoRI and HindIII/PstI respectively digested the rDNA-ITS2-PCR products,except for Meloidogyne javanica. It is evident that this method of rDNA-ITS2-RFLP is useful for the identification of different species of nematodes." @default.
- W2366059897 created "2016-06-24" @default.
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- W2366059897 date "2006-01-01" @default.
- W2366059897 modified "2023-09-22" @default.
- W2366059897 title "Analysis of the rDNA-sequences from internal transcribed space-3 through RFLP" @default.
- W2366059897 hasPublicationYear "2006" @default.
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