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- W2366117885 abstract "Objective To explore the relation between the resistance to antibiotics and the decrease or lack of the membrane protein of the S.aureus after observing the components of the membrane protein. Methods The identification of MRSA was confirmed by detecting the specific mecA gene of MRSA with PCR and the abduction of MRSA was achieved by agar plate dilution test .The outer membrane protein (OMP) of MRSA was extracted by breaking up cell membrane via ultrasonic, the component of OMP was analysed by SDS-PAGE, and the relative quantity of the OMP of MRSA was measured by the spectrophotometric scanner.Results The membrane protein image of the MRSA was in accordance with that of the S.aureus ATCC25923 basically. Compared with VRSA, MRSA was clearer than VRSA for the protein strips whose molecular weights are 45KD,44KD,30KD,20KD,16KD, and 14KD respectively, which suggested that the relative quantities of the 45KD and the 14KD OMP of MRSA were more than those of VRSA. Conclusions The relative quality of the 14KD OMP of VRSA was less than that of MRSA. In addition, the relative quality of the 45KD OMP of VRSA was less than that of MRSA, which suggested that the decrease of the certain protein including 14KD OMP and the 45KD OMP of VRSA was related to one of the mechanisms of vancomycin- resistant of VRSA." @default.
- W2366117885 created "2016-06-24" @default.
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- W2366117885 date "2006-01-01" @default.
- W2366117885 modified "2023-09-28" @default.
- W2366117885 title "Study of the Relation Between the Decrease of the Certain Protein of VRSA and Vancomycin-resistant of VRSA" @default.
- W2366117885 hasPublicationYear "2006" @default.
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