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- W2366136040 abstract "Adult mouse seminiferous tubules and whole testes were slowly frozen in different freezing solutions, stored in liquid nitrogen, thawed in a 37 ℃ water bath, and the cell recovery rates were examined by trypan blue exclusion staining after thawing, respectively. The freezing solutions were DMEM 100 g/L NBS supplemented with 50 mL/L, 100 mL/L, or 150 mL/L dimethyl sulphoxide (DMSO), propanediol(PG), ethylene glycol(EG), or glycerol(G),respectively. The cell recoveries of adult mouse seminiferous tubules in freezing solutions with DMSO at concentrations of 50 mL/L, 100 mL/L and 150 mL/L were 92.6%, 93.2%, and 69.4%, respectively. The cell recoveries in freezing solutions with PG at concentrations of 50 mL/L, 100 mL/L and 150 mL/L were 94.2%, 93.3%, and 66.9%, respectively. The cell recoveries in freezing solutions with EG at concentrations of 50 mL/L, 100 mL/L and 150 mL/L were 87.2%, 91.0%, and 62.6%, respectively. The cell recoveries in freezing solutions with G at concentrations of 5%, 10% and 15% were 90.0%, 90.5%, and 67.1%, respectively. There were no significant differences among the highest recoveries of four cryoprotectants. The results suggest that four cryoprotectaots all are suitable for cryopreservation of adult mouse seminiferous tubules, and their favorable concentration all are at 50 mL/L~100 mL/L. The cell recoveries of adult mouse testes are all below 60%, and further research is needed." @default.
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- W2366136040 title "Cryopreservation of Adult Mouse Seminiferous Tubules and Whole Testes" @default.
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