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- W2366154449 abstract "A cDNA fragment encoding nitrate reductase(NR) was amplified by reverse polymerase chain reaction(RT-PCR) with total RNA extracted from the leaf of cucumber.Sequencing and homology analysis revealed that the gene fragment was 395 bp in length and shared up to 89% similarity with NR gene from winter squash.The deduced amino acid sequence for the fragment was composed of 131 amino acid residues.Further analysis of changes in enzymatic activity and transcript level demonstrated that NR expression and activity declined significantly under nitrogen deficient condition.The results suggested that the cucumber NR activity was regulated at the mRNA level." @default.
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- W2366154449 date "2007-01-01" @default.
- W2366154449 modified "2023-09-23" @default.
- W2366154449 title "Cloning of a cDNA fragment of nitrate reductase(NR) gene in cucumber and its expression analysis under nitrogen deficiency stress" @default.
- W2366154449 hasPublicationYear "2007" @default.
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