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- W2366155704 abstract "The experiment aimed to study the effects of recombinant Ghrelin plasmid on growth performance and stomach weight of rats.Ghrelin coding region was amplified from the gastric fundus mucosa of weaned piglets and cloned into the vector pcDNA3 to construct an expression vector named pcDNA3-Ghrelin.Twelve weaned rats were divided randomly into two groups.100 μg pcDNA3-Ghrelin was injected intramuscularly into rats as treatment,and 100 μg empty plasmid as control.Body weight and feed consumption were recorded on 7 d,14 d and 29 d after injection,respectively.The rats were killed at 29 d to get weight and pH value of stomach.Compared to control,daily gain of rat in treatment group was increased on 7 d and 29 d after injection,feed consumption was not significantly different,feed conversion ratio was reduced,stomach weight and relative index(stomach weigh/body weight) was not significantly different,and stomach pH value declined.These results suggested that intramuscular injection of pcDNA3-Ghrelin could improve growth development of weaned rats." @default.
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- W2366155704 date "2013-01-01" @default.
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- W2366155704 title "Expression of recombinant Ghrelin plasmid and its effects on growth performance and stomach weight of rats" @default.
- W2366155704 hasPublicationYear "2013" @default.
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