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- W2366167044 abstract "Objective To explore a rapid method for early detection of Haemophilus in fluenzae.Methods A pair of primers was designed according to the P6 gene encoding outer membrane protein of Haemophilus influenzae.The specific probes were synthesized by polymerase chain reaction.Bacterium DNA labeled with biotin in sputum specimens was detected by reverse Dot-blot hybridization.Results Only a 351 bp DNA fragment was amplified from haemophilus influenzae.The probes can detect as low as 10 ng of bacterium DNA.No cross hybridization with the DNA of other bacteria, viruses and fungi occurred.Fifty sputum specimens were identified by the developed method and culture simultaneously and the positive rates were 30% and 22% respectively.Conclusion The method is of value in rapid and specific identification of haemophilus influenzae." @default.
- W2366167044 created "2016-06-24" @default.
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- W2366167044 date "2004-01-01" @default.
- W2366167044 modified "2023-09-23" @default.
- W2366167044 title "Detection of Haemophilus influenzae by reverse dot-blot hybridization" @default.
- W2366167044 hasPublicationYear "2004" @default.
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