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- W2366184521 abstract "Objective To purify the immunoglobulin from argus snakehead fish,and to prepare the rabbit polyclonal anti-sera.Methods Argus snakehead fish immunoglobulin was purified using staphylococcal protein A affinity chromatography.The products were examined by SDS-PAGE electrophoresis.Rabbit anti-sera were prepared by repeatedly immunizing rabbits with the purified IgM.And anti-argus snakehead fish IgM antibodies in rabbit sera were detected by agarose double immunodiffusion.Results Immunoglobulin purified from argus snakehead fish had only two bands in SDS-PAGE,and the molecular weight of heavy chain and light chain were about 78×10~3 and 27×10~3 respectively.The titer of rabbit anti-sera obtained was up to 1∶32 examined by agarose double immunodiffusion.Conclusions Argus snakehead fish immunoglobulin was successfully purified,and rabbit antisera were prepared.That might provide a basis for the study on immune mechanism and developing the serological test system of Argus snakehead fish." @default.
- W2366184521 created "2016-06-24" @default.
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- W2366184521 date "2007-01-01" @default.
- W2366184521 modified "2023-09-26" @default.
- W2366184521 title "Purification of Serum Immunoglobulin from Argus Snakehead Fish(Ophicephalus argus) and Preparation of Rabbit Polyclonal Antibody for Argus Snakehead Fish" @default.
- W2366184521 hasPublicationYear "2007" @default.
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