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- W2366230056 abstract "Objective:Applying Porphyromonas gingivalis immunizing hen to induce specific IgY production,and to observe the IgY efficacy value against Porphyromonas gingivalis.Methods:Using immunization,water-dilution,ammonium sulfate precipitation,ELISA,and inhibiting bacteria growth test in liquid anaerobic culture,the IgY were induced,extracted,purified,and the IgY efficacy value against Porphyromonas gingivalis was observed.Results:The IgY purity reached to 87.6%-89.1% after saturation ammonium sulfate precipitation.The IgY efficacy value against Porphyromonas gingivalis was 1∶1600/mg protein.The IgY efficacy value against Capnocytophaga gingivalis,Prevotella intermedia,Actinobacillus actinomycetemcomitans and Fusobacterium nucleatum were 1∶800,1∶800,1∶6 400,1∶12 800 /mg protein in cross-reactivity,respectively.When the IgY concentrations of anti-Porphyromonas gingivalis were 5.0,1.0,0.1 mg/ml,the IgY was cultured separately with Porphyromonas gingivalis,Capnocytophaga gingivalis,Actinobacillus actinomycetemcomitans,Prevotella intermedia,Fusobacterium nucleatum,Actinomyces viscosus and Streptococcus mutans in the 1×108 CFU/L and 5×108 CFU/L for 24 h and 72 h.The growth of these suspected pathogens of periodontal disease was inhibited in various degrees.Conclusion:The hen is able to be induced to produce the IgY of anti-Porphyromonas gingivalis by Porphyromonas gingivalis.The specific IgY of anti-Porphyromonas gingivalis is capable of inhibiting the growth of Porphyromonas gingivalis and the other suspected pathogens of periodontal diseases.There are common antigens between Porphyromonas gingivalis and the other suspected pathogens of periodontal diseases,and there are cross immunizing reactivity between them.It may have the prospect of periodontal disease prevention and treatment." @default.
- W2366230056 created "2016-06-24" @default.
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- W2366230056 date "2010-01-01" @default.
- W2366230056 modified "2023-09-24" @default.
- W2366230056 title "Preparation of IgY against Porphyromonas gingivalis and growth suppression of the suspected pathogens of periodontal disease by IgY against Porphyromonas gingivalis" @default.
- W2366230056 hasPublicationYear "2010" @default.
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