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- W2366272220 abstract "Objective:To modify the ns1 gene of avian influenza virus H5N1 subtype(A/Qa/ST/852/01)and to construct the recombinant expression vector. Methods:The ns1 gene of A/Qa/ST/852/01 was amplified by RT-PCR and a deletion of 15 nucleotides (acid) was inserted into ns1 gene by multiply PCR, then cloned into expression vector pcDNA 3.1(+).Results:The sequence analysis demonstrated that the ns1 gene of A/Qa/ST/852/01 contained one open rea-(ding) frame of 678 bp, which encoded polypeptides of 225 amino acids. The recombinant plasmid of pcDNA 3.1/ns1 was digested with restriction endonuclease and DNA sequence analysis. Conclusion:The deletion of 15 nucleotides acid is successfully inserted into the ns1 gene of A/Qa/ST/852/01,and the construction of the eukaryotic expression vector provides a foundation for further studies on the function of NS1 protein." @default.
- W2366272220 created "2016-06-24" @default.
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- W2366272220 date "2005-01-01" @default.
- W2366272220 modified "2023-09-26" @default.
- W2366272220 title "Modification of ns1 Gene of H5N1 Avian Influenza Virus" @default.
- W2366272220 hasPublicationYear "2005" @default.
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