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- W2366333449 abstract "In this study,the chicken Mx promoter(Mxp)was cloned,and luciferase(luc) gene was inserted into downstream of Mxp.Then pMxpluc was constructed for the bioactivity assay of type Ⅰ IFN and the assay was developed and described as follows.Chicken embryo fibroblast cell line(DF-1) was transfected with pMxp-luc.24 h later,the cells were incubated with 10-fold diluted chicken IFN-α and IFN-β for another 6 h.The expression of luciferase was stimulated by Mxp and had a good linear correlation with the antiviral activity of IFN.This new method was 10-and 100-fold more sensitive than the antiviral assay to quantify chicken IFN-α and IFN-β,respectively.In conclusion,the reporter gene assay described here is more rapid,safe and accurate than antiviral assay,and has potential value for high-flux assay and great value in the fundamental and application study of avian and mammalian type Ⅰ IFN." @default.
- W2366333449 created "2016-06-24" @default.
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- W2366333449 date "2008-01-01" @default.
- W2366333449 modified "2023-09-26" @default.
- W2366333449 title "Mx Promoter-Luciferase Reporter Gene Assay for the Quantification of Chicken Type I Interferon" @default.
- W2366333449 hasPublicationYear "2008" @default.
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