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- W2366335939 abstract "BALB/c mice were immunized with foot-and-mouth disease virus(FMDV) nonstructural protein 3A expressed in Escherichia coli.Splenocytes of the BALB/c mice were fused with myeloma cells SP2/0 for producing hybridoma.Specific antibody was determined by indirect ELISA and positive hybridoma cells were cloned by limiting dilution assay.Three hybridoma cell lines stably secreting monoclonal antibodies(McAbs) against the protein 3A were developed and named 3H2,4B1 and 7F5,respectively.The McAb 7F5 was classified into subtype IgG2b,while 3H2 and 4B1 were identified to be subtype IgG2a.7F5 could recognize different epitope from the other strains.All the McAbs could react with FMDV in IFA.The result of indirect ELISA and Western-blot showed that these McAbs bound specifically to the protein 3A.It was concluded from the above-mentioned result that the prepared McAbs against the protein 3A could not only provide a foundation for studying the structure and function of FMDV,but also a mean for development of diagnostic methods." @default.
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- W2366335939 date "2010-01-01" @default.
- W2366335939 modified "2023-09-26" @default.
- W2366335939 title "Preparation and identification of monoclonal antibodies against nonstructural protein 3A of foot-and-mouth disease virus" @default.
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