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- W2366413708 abstract "Cold-regulated 413-plasma membrane (COR413-PM) proteins are potentially targeted to the plasma membrane, associated with the development of freezing tolerance needed for winter survival in overwintering plants. In attempt to elucidate the molecular mechanism of cold tolerance of wintersweet, which blooms in the deep winter,a COR413 protein gene,designated as Cpcor413pm1,was obtained by sequencing the randomly selected clones,on the basis of Chimonanthus praecox flower cDNA library construction and its ESTs analysis. It was showed that the cDNA of Cpcor413pm1 was 946 bp in length, encoding a predicted protein of 201 amino acids .The CpCOR413PM1 protein contained conserved regions involved in five transmembrane domains (TMDs), one alpha helix which was divided by a G-rich region, one putative glycosylated phosphatidylinositol (GPI)-anchoring site, seven Pro residues and one Cys residue, as well as three phosphorylation sites in Ser residue, Thr residue and Tyr residue, respectively. The N-terminal region of CpCOR413PM1 protein are poorly conserved and no signal peptide was discovered in the protein. Sequence comparisons showed that CpCOR413PM1 was a novel COR413-PM protein from wintersweet. The expression pattern of COR413-PM was analyzed by RT-PCR. It was demonstrated that Cpcor413pm1 gene was expressed in sprout period, flower-bud period, display-petal period, initiating bloom period, bloom period, wither period, and high levels of transcripts were accumulated during initiating bloom period and bloom period,suggesting that Cpcor413pm1 gene was correlated with the development of freezing tolerance in flower of wintersweet." @default.
- W2366413708 created "2016-06-24" @default.
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- W2366413708 date "2006-01-01" @default.
- W2366413708 modified "2023-09-22" @default.
- W2366413708 title "Molecular Characterization and Expression Analysis of a Novel COR413 Gene from Wintersweet[Chimonanthus praecox (L.) Link]" @default.
- W2366413708 hasPublicationYear "2006" @default.
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