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- W2366416837 abstract "The construction of Fc-TNFRII recombinant plasmid expression system by using pEE12.4,FreeStyleTM MAX was transfected into the cells(CHO-cells),the 50mM L-methionine sulfoximine added in the culture medium pressure screening,stable and efficient expression were obtained.Moeover,the culture medium was optimized to additive.Shake flask 200 mL systems were based on the results of the optimal amplification culture.Mabselect SuRe was used to study Fc-TNFRII purification method.The results showed that the high expression cell lines were obtained by screening the final expression level of 58.54 mg/L.The ultimately purity of the Fc-TNFRII 96.5% was obtained by HPLC." @default.
- W2366416837 created "2016-06-24" @default.
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- W2366416837 date "2013-01-01" @default.
- W2366416837 modified "2023-09-25" @default.
- W2366416837 title "CHO Cell Expression Immunoglobulin Constant Regions-tumor Necrosis Factor ReceptorII(Fc-TNFRII)" @default.
- W2366416837 hasPublicationYear "2013" @default.
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