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- W2366485105 abstract "Objective To clone and express the Lentinula edodes C91- 3transcriptome Unigene 28447 and to investigate its biological activity. Methods Lentinula edodes C91- 3mycelium total RNA were extracted. The full- length gene of Lentinula edodes mycelium Unigene 28447 were obtained with 3'- Full RACE,5'- Full RACE kits. The gene expressed in E. coli after induction,then the protein was extracted,purified and refolded. MTT assay and flow cytometry were used to study the tumor cell growth,apoptosis and cell cycle. Results The recombinant protein was successfully induced and expressed in plasmid p ET32a( +). MTT assay showed that different concentrations of this protein inhibited A549 growth significantly as compared with control group( P 0. 05). Flow cytometry showed this protein induced apoptosis of A549 and changed cell cycle significantly( P 0. 05). Conclusion The anti- tumor activity was found in the protein,which would be the basis for further study in the future." @default.
- W2366485105 created "2016-06-24" @default.
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- W2366485105 date "2015-01-01" @default.
- W2366485105 modified "2023-09-25" @default.
- W2366485105 title "Expression of recombination Unigene 28447 of Lentinula edodes C_(91-3) and its effect on apoptosis and cell cycle of lung cancer cell A549" @default.
- W2366485105 hasPublicationYear "2015" @default.
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