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- W2366489481 abstract "Objective To discuss about Se influencing the abilities of macrophages infected by BCG in the fields of NO-producing,survival and treatment of the bacterium in vitro.MethodsThe peritoneal macrophages collected from all groups and Raw264.7 cells were cultivated in various concentrations of selenium. The levels of nitrogen monoxidum(NO)released by Mφ after infection with BCG,which was measured by Griess method. The survival rate was determined by MTT assay and the bacteria burden in Mφ was determined by Ziehl-heelson staining method and colony counting method.ResultsThe levels of NO released by infected Mφ were higher than those without Se holes,specially those released by infected Mφ in the BCG+Se group with 25-100ng/ml Se were significantly different from those without Se holes(P﹤0.05). Meanwhile,survival rates of infected macrophages in RPMI-1640 containing different concentrations of Se were higher than those without Se holes;the bacteria amount in Mφ with Se holes were lower than that without Se holes. However,the results of viable bacteria count in macrophages after swallowing BCG 4 hours were paradoxical.ConclusionThe results suggest that selenium could promote the macrophage’s nature immunologic response and it has a dose-dependent relationship. Meanwhile,Se has a protective function of stress to macrophage." @default.
- W2366489481 created "2016-06-24" @default.
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- W2366489481 date "2008-01-01" @default.
- W2366489481 modified "2023-09-23" @default.
- W2366489481 title "INITIAL DISCUSSION ABOUT ORGANIC SELENIUM STRENGTHENING THE DISPOSAL ABILITY OF MACROPHAGE ON BCG" @default.
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