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- W2366519056 abstract "Objective To prepare and preliminarily identify the monoclonal antibodies(McAbs) against Nsp9 protein of porcine reproductive and respiratory syndrome(PRRSV).Methods BALB / c mice were immunized with purified recombinant Nsp9 protein,based on which McAbs were prepared by hybridoma technique,then analyzed for specificity by Western blot and indirect IFA,and identified for subclass by IgG standard subclass identification kit.The hybridoma cells were resuscitated after storage for 1,2,3 and 4 months respectively and determined for antibody-secreting ability by ELISA.Results Three hybridoma cell strains stably secreting specific McAbs,named as 2D6,3C11 and 4E6,were obtained,by which the secreted McAbs reached ELISA titers of 1 ∶ 6 400,1 ∶ 3 200 and 1 ∶ 1 600 in culture supernatant,and 1 ∶ 640 000,1 ∶ 512 000 and 1 ∶ 256 000 in ascites,respectively.All the McAbs secreted by three strains showed specific binding to Nsp9 and PRRSV.The McAbs secreted by 2D6 and 3C11 strains were IgG1,while that by 4E6 strain were IgM.All the three strains secreted McAbs stably after storage for 4 months.Conclusion The McAbs against PRRSV Nsp9 were prepared,and showed high specificity and stability,which provided a material for development of method for identification of natural infection and vaccine-associated infection of PRRSV." @default.
- W2366519056 created "2016-06-24" @default.
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- W2366519056 date "2012-01-01" @default.
- W2366519056 modified "2023-09-25" @default.
- W2366519056 title "Preparation and preliminary identification of monoclonal antibodies against Nsp9 protein of porcine reproductive and respiratory syndrome virus" @default.
- W2366519056 hasPublicationYear "2012" @default.
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