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- W2366527583 abstract "Objective Cytokeratin-7(Krt7) is closely related to tumor progression and inflammatory response,and in this process,oxidative stress plays an important role.By constructing a eukaryotic expression vector of Krt7,the authors observed the expression and localization of Krt7 and its changes under oxidative stress in NIH 3T3 cells.Methods Total RNA was extracted from the lung tissue of C57 / BL6 mice,and the corresponding coding sequences of mouse Krt7 were amplified by RT-PCR and then cloned into the hemagglutinin(HA)-tagged vector pcDNA3-HA to construct a new recombinant plasmid named pcDNA3-Krt7-HA.The NIH 3T3 cells were then transfected with liposome.The expression of Krt7 was determined by Western blot.The distribution and changes of the expressed products induced by hydrogen peroxide in the transfected NIH 3T3 cells were observed by immunofluorescence.Results The recombinant plasmid was correctly constructed,and it was found to be effectively expressed in the NIH 3T3 cells and distributed mainly in the cytoplasm.With the increased time of H2O2 stimulation,intracellular granular protein aggregates became more apparent and were translocated from cytoplasm to the nucleus at 60 min.And this process was obviously inhibited by the inhibitor of the MEK signaling pathway PD98059.Conclusion The eukaryotic expression vector of the Krt7-HA fusion protein was successfully constructed and effectively expressed in mammalian cells with a correct localization.The morphology and localization of Krt7 in NIH 3T3 cells induced by H2O2depend on the MEK signaling pathway." @default.
- W2366527583 created "2016-06-24" @default.
- W2366527583 creator A5016655840 @default.
- W2366527583 date "2013-01-01" @default.
- W2366527583 modified "2023-09-23" @default.
- W2366527583 title "Effects of MEK signaling pathway on the expression and localization of cytokeratin-7 in NIH 3T3 cells induced by H_2O_2" @default.
- W2366527583 hasPublicationYear "2013" @default.
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