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- W2366572464 abstract "Based on double orthogonal optimum design,using Boehmeria Jacq.DNA as template,the factors influencing RAPD were analyzed.PCR system for RAPD in Boehmeria Jacq.was established: 25 μL reaction solution contained 0.75 μmol/L random primers,150 ng DNA template,2.1 mmol/L Mg2+,0.2 μmol/L dNTP,1 U Taq polymerase.The suitable procedure is one cycle 94 ℃ for 4 min;denaturing at 94 ℃ for 45 s;primer annealing at 37 ℃ for 45 s,extension at 72 ℃ for 90 s,36 cycles;extension at 72 ℃ for 5 min at last and then remaing at 4 ℃.By inspection,the reaction systems were suitable for Boehmeria Jacq.from multiple species." @default.
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- W2366572464 date "2010-01-01" @default.
- W2366572464 modified "2023-09-28" @default.
- W2366572464 title "Double Orthogonal Optimization for RAPD-PCR Reaction System in Boehmeria Jacq." @default.
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