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- W2366602009 abstract "[Objective] To explore the culture condition and differentiation of embryonic stem cells(ESCs)-derived neural stem cells(NSCs) and hippocampus-derived NSCs in vitro. [Methods] Embryonic bodies induced by retinoic acid and retinal Miller cells were selected in NSCs-defined medium. So did cells isolated from hippocampus of 60 E18 BALB/c mice (10 mice in each experiment). The morphological changes and ability of expansion and differentiation of cells from two different origins were comparatively analyzed. Nestin antigen and BrdU incorporation were detected in the selected cells by immunocytochemistry and RT-PCR. [Results] Large amount of neuro-spheres were derived from both embryonic body and hippocampus in the selected medium, which could be passaged and differentiated, stained positive with Nestin and BrdU, and expressed Nestin gene. The percentage of NF positive cells obtained from differentiation of ESCs-derived NSCs was 42.1% ±3.6%, which was significantly higher than that from hippocampus-derived NSCs 18.7% ±5. 1%, P 0.01. [Conclusion] NSCs derived from ESCs have potentially much more powerful ability than hippocampus-derived NSCs to expand and differentiate into neurons, and would play a new alternative role in stem cell transplantation to treat neuropathy of glaucoma and other retinal diseases." @default.
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- W2366602009 date "2003-01-01" @default.
- W2366602009 modified "2023-09-28" @default.
- W2366602009 title "Comparative Study on Culture and Differentiation of Neural Stem Cells Derived from Embryonic Stem Cells and Hippocampus" @default.
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